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【题名】：Anticancer activity of natural and synthetic acetylenic lipids【作者】：Valery M. Dembitsky
【杂志名全称】：Lipids
【年, 卷(期), 起止页码】：2006,Volume 41, Number 10:883-924
【DOI】：10.1007/s11745-006-5044-3
【全文链接】：[url]https://springerlink.metapress.com/content/l778873u22265lr4/resource-secured/?target=fulltext.pdf&sid=xbc3xv55czoame55bia1pi45&sh=www.springerlink.com[/url]
【摘要信息】：This review is a comprehensive survey of acetylenic lipids and their derivatives, obtained from living organisms, that have anticancer activity. Acetylenic metabolites belong to a class of molecules containing triple bond(s). They are found in plants, fungi, microorganisms, and marine invertebrates. Although acetylenes are common as components of terrestrial plants, fungi, and bacteria, it is only within the last 30 years that biologically active polyacetylenes having unusual structural features have been reported from plants, cyanobacteria, algae, invertebrates, and other sources. Naturally occurring aquatic acetylenes are of particular interest since many of them display important biological activities and possess antitumor, antibacterial, antimicrobial, antifouling, antifungal, pesticidal, phototoxic, HIV-inhibitory, and immunosuppressive properties. There is no doubt that they are of great interest, especially for the medicinal and/or pharmaceutical industries. This review presents structures and describes cytotoxic and anticancer activities only for more than 300 acetylenic lipids and their derivatives isolated from living organisms.
【求助者email】：[email]qiudy@caf.ac.cn[/email]
【题名】：An S-acylation switch of conserved G-domain cysteines is required for polarity signaling by ROP GTPases
【作者】：Nadav Sorek, Oshik Segev, Orit Gutman, Einat Bar, Sandra Richter, Limor Poraty, Joel A. Hirsch, Yoav I. Henis, Efraim Lewinsohn, Gerd Jürgens and Shaul Yalovsky
【杂志名全称】：Current Biology
【年, 卷(期), 起止页码】：2010, 20,10:914-920
【DOI】：doi:10.1016/j.cub.2010.03.057
【全文链接】：[url]http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VRT-50160C3-3-J&_cdi=6243&_user=1252940&_pii=S0960982210003830&_orig=search&_coverDate=05%2F25%2F2010&_sk=999799989&view=c&wchp=dGLbVlW-zSkWA&md5=72a70619510503bca38304b891922960&ie=/sdarticle.pdf[/url]
【摘要信息】：Rho GTPases are master regulators of cell polarity [1]. For their function, Rhos must associate with discrete plasma membrane domains [2]. Rho of Plants (ROPs) or RACs comprise a single family [[3], [4] and [5]]. Prenylation and S-acylation of hypervariable domain cysteines of Ras and Rho GTPases are required for their function [[6], [7], [8], [9], [10] and [11]]; however, lipid modifications in the G domain have never been reported. Reversible S-acylation involves the attachment of palmitate (C16:0) or other saturated lipids to cysteines through a thioester linkage and was implicated in the regulation of signaling [12]. Here we show that transient S-acylation of Arabidopsis AtROP6 takes place on two conserved G domain cysteine residues, C21 and C156. C21 is relatively exposed and is accessible for modification, but C156 is not, implying that its S-acylation involves a conformational change. Fluorescence recovery after photobleaching beam-size analysis [13] shows that S-acylation of AtROP6 regulates its membrane-association dynamics, and detergent-solubilization studies indicate that it regulates AtROP6 association with lipid rafts. Site-specific acylation-deficient AtROP6 mutants can bind and hydrolyze GTP but display compromised effects on polar cell growth, endocytic uptake of the tracer dye FM4-64, and distribution of reactive oxygen species. These data reveal an S-acylation switch that regulates Rho signaling.
【求助者email】：[email]qiudy@caf.ac.cn[/email]
【题名】：Protein–Lipid Modifications and Targeting of ROP/RAC and Heterotrimeric G Proteins
【作者】：Nadav Sorek and Shaul Yalovsky
【杂志名全称】： G Protein Signaling in Plants
【年, 卷(期), 起止页码】：71-90
【DOI】：DOI: 10.1007/978-3-642-03524-1_4
【全文链接】：[url]https://springerlink.metapress.com/content/rm4677321007ng02/resource-secured/?target=fulltext.pdf&sid=xbc3xv55czoame55bia1pi45&sh=www.springerlink.com[/url]
【摘要信息】：NA
【求助者email】：[email]qiudy@caf.ac.cn[/email]
【题名】：Analysis of protein S-acylation by gas chromatography|[ndash]|coupled mass spectrometry using purified proteins
【作者】：Nadav Sorek and Shaul Yalovsky
【杂志名全称】：Nature Protocols
【年, 卷(期), 起止页码】：2010,5, 834–840
【DOI】：doi:10.1038/nprot.2010.33
【全文链接】：[url]http://www.nature.com/nprot/journal/v5/n5/pdf/nprot.2010.33.pdf[/url]
【摘要信息】：S-acylation, also known as palmitoylation, involves the attachment of acyl fatty acids to thiol groups of cysteine residues through a reversible thioester bond. Owing to its reversibility, S-acylation is important in regulation of diverse signaling cascades, including Ras-associated cancers in mammals, stress response and metabolic regulation. Here we describe a simple protocol for analysis of protein S-acylation using gas chromatography–coupled mass spectrometry. Analysis can be carried out with as little as 1 |[mu]|g of purified protein and allows chemical identification and, potentially, quantification of the acyl moieties. The method is based on cleavage of the fatty acids from proteins by hydrogenation with platinum (IV) oxide. This causes an acid transesterification of the acyl groups, adding an ethyl group to the carboxyl head of the fatty acid. The addition of the ethyl group reduces the polarity of the fatty acids, allowing their efficient separation by gas chromatography.
【求助者email】：[email]qiudy@caf.ac.cn[/email]
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